hotspot
A tool for identifying informative genes (and gene modules) in a single-cell dataset.
Two fundamental aims that emerge when analyzing single-cell RNA-seq data are identifying which genes vary in an informative manner and determining how these genes organize into modules. Hotspot is a tool for identifying informative genes (and gene modules) in a single-cell dataset. Hotspot identifies these ‘informative’ genes based on how well a gene’s variation agrees with some cell metric - some similarity mapping between cells. Genes which are informative are those whose expression varies in similar way among cells which are nearby in the given metric.
You can view and install the tool from GitHub. For a more thorough description of the method, refer to our publication.